PrimePCR™ SYBR® Green Assay: ACHE, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013635
Assay Design:   Intron-spanning
Chromosome Location:   7:100488867-100490045question
Amplicon Length:   154
Splice Variants Targeted:   ENST00000419336 ENST00000241069 ENST00000428317 ENST00000412389 ENST00000411582 ENST00000302913 ENST00000422451 ENST00000447483

Gene Information

Acetylcholinesterase hydrolyzes the neurotransmitter acetylcholine at neuromuscular junctions and brain cholinergic synapses and thus terminates signal transmission. It is also found on the red blood cell membranes where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene and the structural diversity in the gene products arises from alternative mRNA splicing and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain muscle and other tissues is the hydrophilic species which forms disulfide-linked oligomers with collagenous or lipid-containing structural subunits. The other alternatively spliced form expressed primarily in the erythroid tissues differs at the C-terminal end and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq Jul 2008]

Gene Symbol:   ACHE
Gene Name:   acetylcholinesterase
Aliases:   ARACHE, N-ACHE, YT
RefSeq:   NC_000007.13 NG_007474.1 NT_007933.15
Ensembl:   ENSG00000087085
Entrez:   43
UniGene:   Hs.154495
Chromosome Mapping:   7q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.390000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download