PrimePCR™ SYBR® Green Assay: CYP17A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028954

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013624
Assay Design:   Intron-spanning
Chromosome Location:   10:104592923-104594630question
Amplicon Length:   189
Splice Variants Targeted:   ENST00000369887

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 1720-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins mineralocorticoids glucocorticoids androgens and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency 17-alpha-hydroxylase/1720-lyase deficiency pseudohermaphroditism and adrenal hyperplasia. [provided by RefSeq Jul 2008]

Gene Symbol:   CYP17A1
Gene Name:   cytochrome P450, family 17, subfamily A, polypeptide 1
Aliases:   CPT7, CYP17, P450C17, S17AH
RefSeq:   NC_000010.10 NG_007955.1 NT_030059.13
Ensembl:   ENSG00000148795
Entrez:   1586
UniGene:   Hs.438016
Chromosome Mapping:   10q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.220000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download