PrimePCR™ SYBR® Green Assay: PLIN2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013507
Assay Design:   Intron-spanning
Chromosome Location:   9:19123586-19126213question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000276914 ENST00000434144 ENST00000380465 ENST00000380464 ENST00000411567

Gene Information

The protein encoded by this gene belongs to the perilipin family members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material and maybe involved in development and maintenance of adipose tissue. However it is not restricted to adipocytes as previously thought but is found in a wide range of cultured cell lines including fibroblasts endothelial and epithelial cells and tissues such as lactating mammary gland adrenal cortex Sertoli and Leydig cells and hepatocytes in alcoholic liver cirrhosis suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Mar 2011]

Gene Symbol:   PLIN2
Gene Name:   perilipin 2
Aliases:   ADFP, ADRP, MGC10598
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000147872
Entrez:   123
UniGene:   Hs.3416
Chromosome Mapping:   9p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.750000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download