This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: PLIN2, Human
PrimePCR™ Template for SYBR® Green Assay: PLIN2, Human
The protein encoded by this gene belongs to the perilipin family members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material and maybe involved in development and maintenance of adipose tissue. However it is not restricted to adipocytes as previously thought but is found in a wide range of cultured cell lines including fibroblasts endothelial and epithelial cells and tissues such as lactating mammary gland adrenal cortex Sertoli and Leydig cells and hepatocytes in alcoholic liver cirrhosis suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Mar 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.