PrimePCR™ SYBR® Green Assay: H6PD, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028881

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013468
Assay Design:   Intron-spanning
Chromosome Location:   1:9322289-9323645question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000377403

Gene Information

There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form encoded by this gene is autosomally linked. This H form shows activity with other hexose-6-phosphates especially galactose-6-phosphate whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues but H form is not found in red cells. [provided by RefSeq Jul 2008]

Gene Symbol:   H6PD
Gene Name:   hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)
Aliases:   DKFZp686A01246, G6PDH, GDH, MGC87643
RefSeq:   NC_000001.10 NG_012218.1 NT_021937.19
Ensembl:   ENSG00000049239
Entrez:   9563
UniGene:   Hs.463511
Chromosome Mapping:   1p36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.990000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download