This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028881
PrimePCR™ PreAmp for SYBR® Green Assay: H6PD, Human
PrimePCR™ Template for SYBR® Green Assay: H6PD, Human
There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form encoded by this gene is autosomally linked. This H form shows activity with other hexose-6-phosphates especially galactose-6-phosphate whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues but H form is not found in red cells. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.