This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028879
PrimePCR™ PreAmp for SYBR® Green Assay: FETUB, Human
PrimePCR™ Template for SYBR® Green Assay: FETUB, Human
The protein encoded by this gene is a member of the fetuin family part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions including osteogenesis and bone resorption regulation of the insulin and hepatocyte growth factor receptors and response to systemic inflammation. This protein may be secreted by cells. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.