PrimePCR™ SYBR® Green Assay: CSF2RA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013436
Assay Design:   Intron-spanning
Chromosome Location:   X:1404749-1407468question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000381529 ENST00000381524 ENST00000412290 ENST00000419094 ENST00000381509 ENST00000355805 ENST00000355432 ENST00000381500 ENST00000432318 ENST00000417535 ENST00000361536 ENST00000381507

Gene Information

The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2 a cytokine which controls the production differentiation and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene with some of the isoforms being membrane-bound and others being soluble. [provided by RefSeq Jul 2008]

Gene Symbol:   CSF2RA
Gene Name:   colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
Aliases:   CD116, CDw116, CSF2R, CSF2RAX, CSF2RAY, CSF2RX, CSF2RY, GM-CSF-R-alpha, GMCSFR, GMR, MGC3848, MGC4838
RefSeq:   NC_000023.10 NC_000024.9 NT_167196.1 NT_167204.1 NG_012280.1
Ensembl:   ENSG00000198223
Entrez:   1438
UniGene:   Hs.520937
Chromosome Mapping:   Xp22.32 and Yp11.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.670000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download