This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028850
PrimePCR™ PreAmp for SYBR® Green Assay: POU2F1, Human
PrimePCR™ Template for SYBR® Green Assay: POU2F1, Human
The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al. 1993 [PubMed 8314572]). Members of this family contain the POU domain a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM Jul 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.