PrimePCR™ SYBR® Green Assay: ODF2L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013353
Assay Design:   Intron-spanning
Chromosome Location:   1:86842047-86848000question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000459999 ENST00000359242 ENST00000460698 ENST00000294678 ENST00000441121 ENST00000370566 ENST00000317336 ENST00000370567 ENST00000394731

Gene Information

Description Not Available

Gene Symbol:   ODF2L
Gene Name:   outer dense fiber of sperm tails 2-like
Aliases:   KIAA1229, MGC111060, RP5-977L11.1, dJ977L11.1
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000122417
Entrez:   57489
UniGene:   Hs.149360
Chromosome Mapping:   1p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download