PrimePCR™ SYBR® Green Assay: TPO, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028825

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013326
Assay Design:   Intron-spanning
Chromosome Location:   2:1426824-1437301question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000382269 ENST00000329066 ENST00000382201 ENST00000423320 ENST00000382198 ENST00000337415 ENST00000345913 ENST00000346956 ENST00000349624 ENST00000539820 ENST00000536482

Gene Information

This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis including congenital hypothyroidism congenital goiter and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene but the full-length nature of some variants has not been determined. [provided by RefSeq May 2011]

Gene Symbol:   TPO
Gene Name:   thyroid peroxidase
Aliases:   MSA, TDH2A, TPX
RefSeq:   NC_000002.11 NG_011581.1 NT_022221.13
Ensembl:   ENSG00000115705
Entrez:   7173
UniGene:   Hs.467554
Chromosome Mapping:   2p25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.150000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download