This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028823
PrimePCR™ PreAmp for SYBR® Green Assay: PFKL, Human
PrimePCR™ Template for SYBR® Green Assay: PFKL, Human
Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis namely the conversion of D-fructose 6-phosphate to D-fructose 16-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits PFK from liver is a homotetramer of L-subunits while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq Aug 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.