This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: ADH7, Human
PrimePCR™ Template for SYBR® Green Assay: ADH7, Human
This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates including ethanol retinol other aliphatic alcohols hydroxysteroids and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver thus differing from the other known gene family members. Alternative splicing results in multiple transcript variants. [provided by RefSeq Oct 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.