This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: RALBP1, Human
PrimePCR™ Template for SYBR® Green Assay: RALBP1, Human
RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins such as RAL have GDP-bound inactive and GTP-bound active forms which shift from the inactive to the active state through the action of RALGDS (MIM 601619) which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig 2003 [PubMed 12888294]).[supplied by OMIM Nov 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.