This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028742
PrimePCR™ PreAmp for SYBR® Green Assay: OBFC2B, Human
PrimePCR™ Template for SYBR® Green Assay: OBFC2B, Human
Single-stranded DNA (ssDNA)-binding proteins such as OBFC2B are ubiquitous and essential for a variety of DNA metabolic processes including replication recombination and detection and repair of damage (Richard et al. 2008 [PubMed 18449195]).[supplied by OMIM Jun 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.