This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: RAB11B, Human
PrimePCR™ Template for SYBR® Green Assay: RAB11B, Human
The Ras superfamily of small GTP-binding proteins which includes the Ras (see MIM 190020) Ral (see MIM 179550) Rho (see MIM 165390) Rap (see MIM 179520) and Rab (see MIM 179508) families is involved in controlling a diverse set of essential cellular functions. The Rab family including RAB11B appears to play a critical role in regulating exocytotic and endocytotic pathways (summary by Zhu et al. 1994 [PubMed 7811277]).[supplied by OMIM Nov 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.