PrimePCR™ SYBR® Green Assay: ATP1B1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028736

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ATP1B1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ATP1B1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013127
Assay Design:   Intron-spanning
Chromosome Location:   1:169080653-169094152question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000367816 ENST00000367815 ENST00000367813 ENST00000499679

Gene Information

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation for sodium-coupled transport of a variety of organic and inorganic molecules and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates through assembly of alpha/beta heterodimers the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity is not known. [provided by RefSeq Mar 2010]

Gene Symbol:   ATP1B1
Gene Name:   ATPase, Na+/K+ transporting, beta 1 polypeptide
Aliases:   ATP1B, MGC1798
RefSeq:   NC_000001.10 NG_023230.1 NT_004487.19
Ensembl:   ENSG00000143153
Entrez:   481
Chromosome Mapping:   1q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.190000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download