This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028721
PrimePCR™ PreAmp for SYBR® Green Assay: IL27RA, Human
PrimePCR™ Template for SYBR® Green Assay: IL27RA, Human
In mice CD4+ helper T-cells differentiate into type 1 (Th1) cells which are critical for cell-mediated immunity predominantly under the influence of IL12. Also IL4 influences their differentiation into type 2 (Th2) cells which are critical for most antibody responses. Mice deficient in these cytokines their receptors or associated transcription factors have impaired but are not absent of Th1 or Th2 immune responses. This gene encodes a protein which is similar to the mouse T-cell cytokine receptor Tccr at the amino acid level and is predicted to be a glycosylated transmembrane protein. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.