PrimePCR™ SYBR® Green Assay: HTR3E, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: HTR3E, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HTR3E, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012988
Assay Design:   Intron-spanning
Chromosome Location:   3:183818392-183822042question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000415389 ENST00000425359 ENST00000335304 ENST00000431041 ENST00000436361 ENST00000440596

Gene Information

This locus encodes a 5-hydroxytryptamine (serotonin) receptor subunit. The encoded protein subunit E may play a role in neurotransmission in myenteric neurons. Genes encoding subunits C D and E form a cluster on chromosome 3. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq Feb 2012]

Gene Symbol:   HTR3E
Gene Name:   5-hydroxytryptamine (serotonin) receptor 3, family member E
Aliases:   5-HT3c1, MGC120035, MGC120036, MGC120037
RefSeq:   NC_000003.11 NG_012452.1 NT_005612.16
Ensembl:   ENSG00000186038
Entrez:   285242
Chromosome Mapping:   3q27.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.280000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download