This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028619
PrimePCR™ PreAmp for SYBR® Green Assay: IRS2, Human
PrimePCR™ Template for SYBR® Green Assay: IRS2, Human
This gene encodes the insulin receptor substrate 2 a cytoplasmic signaling molecule that mediates effects of insulin insulin-like growth factor 1 and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.