PrimePCR™ SYBR® Green Assay: GINS2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012817
Assay Design:   Intron-spanning
Chromosome Location:   16:85712146-85715232question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000253462

Gene Information

The yeast heterotetrameric GINS complex is made up of Sld5 (GINS4; MIM 610611) Psf1 (GINS1; MIM 610608) Psf2 and Psf3 (GINS3; MIM 610610). The formation of this complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al. 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.[supplied by OMIM Mar 2008]

Gene Symbol:   GINS2
Gene Name:   GINS complex subunit 2 (Psf2 homolog)
Aliases:   HSPC037, PSF2, Pfs2
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000131153
Entrez:   51659
UniGene:   Hs.433180
Chromosome Mapping:   16q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.580000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download