PrimePCR™ SYBR® Green Assay: ERBB2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012766
Assay Design:   Intron-spanning
Chromosome Location:   17:37882058-37882860question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000541774 ENST00000445658 ENST00000269571 ENST00000406381 ENST00000540147

Gene Information

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported with the most common allele Ile654/Ile655 shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers including breast and ovarian tumors. Alternative splicing results in several additional transcript variants some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   ERBB2
Gene Name:   v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Aliases:   CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1
RefSeq:   NC_000017.10 NT_010783.15 NG_007503.1
Ensembl:   ENSG00000141736
Entrez:   2064
UniGene:   Hs.446352
Chromosome Mapping:   17q11.2-q12,17q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 34.000000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download