PrimePCR™ SYBR® Green Assay: EIF2S1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012740
Assay Design:   Intron-spanning
Chromosome Location:   14:67848318-67849201question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000256383 ENST00000557310 ENST00000466499 ENST00000555876

Gene Information

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA EIF2 and GTP. EIF2 is composed of 3 nonidentical subunits the 36-kD EIF2-alpha subunit (EIF2S1) the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908) and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al. 1987 [PubMed 2948954]).[supplied by OMIM Feb 2010]

Gene Symbol:   EIF2S1
Gene Name:   eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Aliases:   EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000134001
Entrez:   1965
UniGene:   Hs.151777
Chromosome Mapping:   14q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.390000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download