This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028545
PrimePCR™ PreAmp for SYBR® Green Assay: STARD5, Human
PrimePCR™ Template for SYBR® Green Assay: STARD5, Human
Cholesterol homeostasis is regulated at least in part by sterol regulatory element (SRE)-binding proteins (e.g. SREBP1; MIM 184756) and by liver X receptors (e.g. LXRA; MIM 602423). Upon sterol depletion LXRs are inactive and SREBPs are cleaved after which they bind promoter SREs and activate genes involved in cholesterol biosynthesis and uptake. Sterol transport is mediated by vesicles or by soluble protein carriers such as steroidogenic acute regulatory protein (STAR; MIM 600617). STAR is homologous to a family of proteins containing a 200- to 210-amino acid STAR-related lipid transfer (START) domain including STARD5 (Soccio et al. 2002 [PubMed 12011452]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.