PrimePCR™ SYBR® Green Assay: STARD5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028545

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012672
Assay Design:   Intron-spanning
Chromosome Location:   15:81610750-81611757question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000302824 ENST00000325346

Gene Information

Cholesterol homeostasis is regulated at least in part by sterol regulatory element (SRE)-binding proteins (e.g. SREBP1; MIM 184756) and by liver X receptors (e.g. LXRA; MIM 602423). Upon sterol depletion LXRs are inactive and SREBPs are cleaved after which they bind promoter SREs and activate genes involved in cholesterol biosynthesis and uptake. Sterol transport is mediated by vesicles or by soluble protein carriers such as steroidogenic acute regulatory protein (STAR; MIM 600617). STAR is homologous to a family of proteins containing a 200- to 210-amino acid STAR-related lipid transfer (START) domain including STARD5 (Soccio et al. 2002 [PubMed 12011452]).[supplied by OMIM Mar 2008]

Gene Symbol:   STARD5
Gene Name:   StAR-related lipid transfer (START) domain containing 5
Aliases:   MGC10327
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000172345
Entrez:   80765
UniGene:   Hs.513075
Chromosome Mapping:   15q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.940000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download