PrimePCR™ SYBR® Green Assay: RAI14, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028535

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012640
Assay Design:   Intron-spanning
Chromosome Location:   5:34796063-34803845question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000265109 ENST00000514527 ENST00000513974 ENST00000512629 ENST00000428746 ENST00000514873 ENST00000503673 ENST00000504052 ENST00000512305 ENST00000514036 ENST00000515799 ENST00000508315 ENST00000512625 ENST00000506376 ENST00000397449

Gene Information

Description Not Available

Gene Symbol:   RAI14
Gene Name:   retinoic acid induced 14
Aliases:   DKFZp564G013, KIAA1334, NORPEG, RAI13
RefSeq:   NC_000005.9 NT_006576.16
Ensembl:   ENSG00000039560
Entrez:   26064
UniGene:   Hs.431400
Chromosome Mapping:   5p13.3-p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.410000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download