PrimePCR™ SYBR® Green Assay: ZMYND8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028526

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012626
Assay Design:   Intron-spanning
Chromosome Location:   20:45875149-45878087question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000360911 ENST00000311275 ENST00000458360 ENST00000467200 ENST00000262975 ENST00000471951 ENST00000352431 ENST00000461685 ENST00000372023 ENST00000540497 ENST00000536340 ENST00000396281 ENST00000355972 ENST00000446994

Gene Information

The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition this protein is a cutaneous T-cell lymphoma-associated antigen. Finally the protein contains a bromodomain and two zinc fingers and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ZMYND8
Gene Name:   zinc finger, MYND-type containing 8
Aliases:   MGC31836, PRKCBP1, PRO2893, RACK7
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000101040
Entrez:   23613
UniGene:   Hs.446240
Chromosome Mapping:   20q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.230000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download