PrimePCR™ SYBR® Green Assay: SLC25A25, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028503

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012568
Assay Design:   Intron-spanning
Chromosome Location:   9:130866085-130868098question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000373068 ENST00000373069 ENST00000432073 ENST00000373066 ENST00000373064 ENST00000433501

Gene Information

Description Not Available

Gene Symbol:   SLC25A25
Gene Name:   solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25
Aliases:   KIAA1896, MCSC, MGC105138, MGC119514, MGC119515, MGC119516, MGC119517, PCSCL, RP11-395P17.4, SCAMC-2
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000148339
Entrez:   114789
UniGene:   Hs.5476
Chromosome Mapping:   9q34.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.120000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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