PrimePCR™ SYBR® Green Assay: ARPP21, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012556
Assay Design:   Intron-spanning
Chromosome Location:   3:35723313-35725274question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000450234 ENST00000428373 ENST00000444190 ENST00000187397 ENST00000452563 ENST00000438577 ENST00000427542 ENST00000474696 ENST00000412048 ENST00000396482 ENST00000432682 ENST00000432450 ENST00000413378 ENST00000417925 ENST00000396481 ENST00000441454 ENST00000436702 ENST00000438071 ENST00000458225 ENST00000337271

Gene Information

The 21-kD cAMP-regulated phosphoprotein (ARPP21) also known as regulator of calmodulin (CaM; MIM 114180) signaling (RCS) plays a central role in integration of key neurotransmitter inputs into medium spiny neurons through its regulation of CaM-dependent kinase I (CaMKI) and protein phosphatase-2B (PP2B) (Rakhilin et al. 2004 [PubMed 15499021]).[supplied by OMIM Feb 2011]

Gene Symbol:   ARPP21
Gene Name:   cAMP-regulated phosphoprotein, 21kDa
Aliases:   ARPP-21, FLJ32997, R3HDM3, RCS, TARPP
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000172995
Entrez:   10777
UniGene:   Hs.475902
Chromosome Mapping:   3p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.790000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download