PrimePCR™ SYBR® Green Assay: TSPAN32, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028478

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012528
Assay Design:   Intron-spanning
Chromosome Location:   11:2329998-2334962question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000182290 ENST00000381121 ENST00000451520

Gene Information

This gene which is a member of the tetraspanin superfamily is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of chromosome 11p15.5 an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome Wilms tumor rhabdomyosarcoma adrenocortical carcinoma and lung ovarian and breast cancers. This gene is located among several imprinted genes; however this gene as well as the tumor-suppressing subchromosomal transferable fragment 4 escapes imprinting. This gene may play a role in malignancies and diseases that involve this region and it is also involved in hematopoietic cell function. Alternatively spliced transcript variants have been described but their biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   TSPAN32
Gene Name:   tetraspanin 32
Aliases:   ART1, FLJ17158, FLJ97586, MGC22455, PHEMX, PHMX, TSSC6
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000064201
Entrez:   10077
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.860000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download