This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028478
PrimePCR™ PreAmp for SYBR® Green Assay: TSPAN32, Human
PrimePCR™ Template for SYBR® Green Assay: TSPAN32, Human
This gene which is a member of the tetraspanin superfamily is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of chromosome 11p15.5 an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome Wilms tumor rhabdomyosarcoma adrenocortical carcinoma and lung ovarian and breast cancers. This gene is located among several imprinted genes; however this gene as well as the tumor-suppressing subchromosomal transferable fragment 4 escapes imprinting. This gene may play a role in malignancies and diseases that involve this region and it is also involved in hematopoietic cell function. Alternatively spliced transcript variants have been described but their biological validity has not been determined. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.