PrimePCR™ SYBR® Green Assay: DLG1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012506
Assay Design:   Intron-spanning
Chromosome Location:   3:196867069-196876616question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000346964 ENST00000419354 ENST00000452595 ENST00000422288 ENST00000448528 ENST00000443183 ENST00000392382 ENST00000450955 ENST00000453607 ENST00000359922 ENST00000357674 ENST00000381807 ENST00000314062

Gene Information

This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation signal transduction cell proliferation synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene but the full-length nature of some of the variants is not known. [provided by RefSeq Feb 2011]

Gene Symbol:   DLG1
Gene Name:   discs, large homolog 1 (Drosophila)
Aliases:   DKFZp761P0818, DKFZp781B0426, DLGH1, SAP-97, SAP97, dJ1061C18.1.1, hdlg
RefSeq:   NC_000003.11 NT_029928.13
Ensembl:   ENSG00000075711
Entrez:   1739
UniGene:   Hs.292549
Chromosome Mapping:   3q29

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.210000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download