This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage
PrimePCR™ PreAmp for SYBR® Green Assay: DLG1, Human
PrimePCR™ Template for SYBR® Green Assay: DLG1, Human
This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation signal transduction cell proliferation synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene but the full-length nature of some of the variants is not known. [provided by RefSeq Feb 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.