PrimePCR™ SYBR® Green Assay: MANSC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012498
Assay Design:   Intron-spanning
Chromosome Location:   12:12483797-12491436question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000535902 ENST00000396349 ENST00000355566 ENST00000545735

Gene Information

Description Not Available

Gene Symbol:   MANSC1
Gene Name:   MANSC domain containing 1
Aliases:   9130403P13Rik, FLJ10298, LOH12CR3
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000111261
Entrez:   54682
UniGene:   Hs.591145
Chromosome Mapping:   12p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.390000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download