This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: GPR85, Human
PrimePCR™ Template for SYBR® Green Assay: GPR85, Human
Members of the G protein-coupled receptor (GPCR) family such as GPR85 have a similar structure characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli such as neurotransmitters hormones or light induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins or G proteins (Matsumoto et al. 2000 [PubMed 10833454]).[supplied by OMIM Aug 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.