PrimePCR™ SYBR® Green Assay: GPR85, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012435
Assay Design:   Intron-spanning
Chromosome Location:   7:112724890-112726107question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000297146 ENST00000424100 ENST00000449591 ENST00000438062 ENST00000501255

Gene Information

Members of the G protein-coupled receptor (GPCR) family such as GPR85 have a similar structure characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli such as neurotransmitters hormones or light induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins or G proteins (Matsumoto et al. 2000 [PubMed 10833454]).[supplied by OMIM Aug 2008]

Gene Symbol:   GPR85
Gene Name:   G protein-coupled receptor 85
Aliases:   FLJ50993, FLJ51159, FLJ99433, SREB, SREB2
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000164604
Entrez:   54329
UniGene:   Hs.152009
Chromosome Mapping:   7q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.230000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download