This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028437
PrimePCR™ PreAmp for SYBR® Green Assay: MEF2C, Human
PrimePCR™ Template for SYBR® Green Assay: MEF2C, Human
This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins which play a role in myogenesis. The encoded protein MEF2 polypeptide C has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation stereotypic movements epilepsy and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq Jul 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.