PrimePCR™ SYBR® Green Assay: CDK5RAP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028422

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012381
Assay Design:   Intron-spanning
Chromosome Location:   20:31958341-31960503question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000427097 ENST00000346416 ENST00000357886 ENST00000339269 ENST00000452723 ENST00000375351

Gene Information

Neuronal CDC2-like kinase which is involved in the regulation of neuronal differentiation is composed of a catalytic subunit CDK5 and an activating subunit p25NCK5A. The protein encoded by this gene binds to p25NCK5A and therefore may be involved in neuronal differentiation. Multiple transcript variants exist for this gene but the full-length natures of only two have been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   CDK5RAP1
Gene Name:   CDK5 regulatory subunit associated protein 1
Aliases:   C20orf34, C42, CDK5RAP1.3, CDK5RAP1.4, CGI-05, HSPC167
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000101391
Entrez:   51654
UniGene:   Hs.435952
Chromosome Mapping:   20pter-q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.790000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download