PrimePCR™ SYBR® Green Assay: AP4M1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012370
Assay Design:   Intron-spanning
Chromosome Location:   7:99701717-99702703question
Amplicon Length:   134
Splice Variants Targeted:   ENST00000438383 ENST00000429084 ENST00000359593 ENST00000439416 ENST00000421755 ENST00000422582

Gene Information

This gene encodes a subunit of the heterotetrameric AP-4 complex. The encoded protein belongs to the adaptor complexes medium subunits family. This AP-4 complex is involved in the recognition and sorting of cargo proteins with tyrosine-based motifs from the trans-golgi network to the endosomal-lysosomal system. [provided by RefSeq Jul 2008]

Gene Symbol:   AP4M1
Gene Name:   adaptor-related protein complex 4, mu 1 subunit
Aliases:   CPSQ3, MU-4, MU-ARP2
RefSeq:   NC_000007.13 NG_016312.1 NT_007933.15
Ensembl:   ENSG00000221838
Entrez:   9179
UniGene:   Hs.632317
Chromosome Mapping:   7q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 33.910000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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