PrimePCR™ SYBR® Green Assay: GPR126, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028407

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012346
Assay Design:   Intron-spanning
Chromosome Location:   6:142718823-142721703question
Amplicon Length:   122
Splice Variants Targeted:   ENST00000230173 ENST00000367608 ENST00000296932 ENST00000367609 ENST00000508295

Gene Information

This gene which is upregulated in human umbilical vein endothelial cells encodes a G protein-coupled receptor. Variations in this gene can affect a person's stature. Multiple transcript variants encoding different proteins have been found for this gene. [provided by RefSeq Mar 2009]

Gene Symbol:   GPR126
Gene Name:   G protein-coupled receptor 126
Aliases:   APG1, DREG, PS1TP2, VIGR
RefSeq:   NC_000006.11 NT_025741.15 NG_011839.1
Ensembl:   ENSG00000112414
Entrez:   57211
UniGene:   Hs.318894
Chromosome Mapping:   6q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.410000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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