This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: SPTLC3, Human
PrimePCR™ Template for SYBR® Green Assay: SPTLC3, Human
The SPTLC3 gene encodes an isoform of the third subunit of serine palmitoyltransferase (SPT; EC 220.127.116.11) which catalyzes the rate-limiting step of the de novo synthesis of sphingolipids (Hornemann et al. 2006 [PubMed 17023427]). SPT contains 2 main subunits: the common SPTLC1 subunit (MIM 605712) and either SPTLC2 (MIM 605713) or its isoform SPTLC2L (SPTLC3) depending on the tissue in which biosynthesis occurs (Hornemann et al. 2006 [PubMed 17023427]). There are also 2 highly related isoforms of a third subunit SSSPTA (MIM 613540) and SSSPTB (MIM 610412) that confer acyl-CoA preference of the SPT enzyme and are essential for maximal enzyme activity (Han et al. 2009 [PubMed 19416851]).[supplied by OMIM Nov 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.