PrimePCR™ SYBR® Green Assay: QKI, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012206
Assay Design:   Intron-spanning
Chromosome Location:   6:163876368-163899882question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000453779 ENST00000275262 ENST00000392127 ENST00000361752 ENST00000361195 ENST00000424802 ENST00000544436 ENST00000537041 ENST00000544823

Gene Information

The protein encoded by this gene is an RNA-binding protein that regulates pre-mRNA splicing export of mRNAs from the nucleus protein translation and mRNA stability. The encoded protein is involved in myelinization and oligodendrocyte differentiation and may play a role in schizophrenia. Four transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Sep 2011]

Gene Symbol:   QKI
Gene Name:   quaking homolog, KH domain RNA binding (mouse)
Aliases:   DKFZp586I0923, Hqk, QK, QK1, QK3
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000112531
Entrez:   9444
UniGene:   Hs.510324
Chromosome Mapping:   6q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.740000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download