PrimePCR™ SYBR® Green Assay: PAX6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028341

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012192
Assay Design:   Intron-spanning
Chromosome Location:   11:31811476-31812330question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000379132 ENST00000379129 ENST00000379107 ENST00000464174 ENST00000241001 ENST00000379115 ENST00000474783 ENST00000379111 ENST00000379123 ENST00000494377 ENST00000470027 ENST00000379109 ENST00000533333 ENST00000419022

Gene Information

This gene encodes paired box gene 6 one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family a conserved paired box domain the encoded protein also contains a homeo box domain. Both domains are known to bind DNA and function as regulators of gene transcription. This gene is expressed in the developing nervous system and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   PAX6
Gene Name:   paired box 6
Aliases:   AN, AN2, D11S812E, MGC17209, MGDA, WAGR
RefSeq:   NC_000011.9 NG_008679.1 NT_009237.18
Ensembl:   ENSG00000007372
Entrez:   5080
UniGene:   Hs.270303
Chromosome Mapping:   11p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.410000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download