PrimePCR™ SYBR® Green Assay: ASH1L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028340

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012189
Assay Design:   Intron-spanning
Chromosome Location:   1:155385677-155408211question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000368346 ENST00000392403

Gene Information

This gene encodes a member of the trithorax group of transcriptional activators. The protein contains four AT hooks a SET domain a PHD-finger motif and a bromodomain. It is localized to many small speckles in the nucleus and also to cell-cell tight junctions. [provided by RefSeq Jul 2008]

Gene Symbol:   ASH1L
Gene Name:   ash1 (absent, small, or homeotic)-like (Drosophila)
Aliases:   ASH1, ASH1L1, FLJ10504, KIAA1420, KMT2H
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000116539
Entrez:   55870
UniGene:   Hs.491060
Chromosome Mapping:   1q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 33.830000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download