PrimePCR™ SYBR® Green Assay: IQGAP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012185
Assay Design:   Intron-spanning
Chromosome Location:   5:75960878-75964558question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000274364 ENST00000505766 ENST00000514001 ENST00000396234 ENST00000502745 ENST00000379730 ENST00000545384

Gene Information

This gene encodes a member of the IQGAP family. The protein contains three IQ domains one calponin homology domain one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton with cell adhesion molecules and with several signaling molecules to regulate cell morphology and motility. [provided by RefSeq Jul 2008]

Gene Symbol:   IQGAP2
Gene Name:   IQ motif containing GTPase activating protein 2
Aliases:   Not Available
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000145703
Entrez:   10788
UniGene:   Hs.291030
Chromosome Mapping:   5q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.710000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download