This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: NME6, Human
PrimePCR™ Template for SYBR® Green Assay: NME6, Human
Nucleoside diphosphate (NDP) kinases (EC 220.127.116.11) such as NME6 are ubiquitous enzymes that catalyze transfer of gamma-phosphates via a phosphohistidine intermediate between nucleoside and dioxynucleoside tri- and diphosphates (Mehus et al. 1999 [PubMed 10453732]).[supplied by OMIM Jul 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.