PrimePCR™ SYBR® Green Assay: XPNPEP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028325

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: XPNPEP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: XPNPEP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012153
Assay Design:   Intron-spanning
Chromosome Location:   10:111642352-111643896question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000502935 ENST00000369683 ENST00000403138 ENST00000423625 ENST00000322238 ENST00000369680

Gene Information

This gene encodes the cytosolic form of a metalloaminopeptidase that catalyzes the cleavage of the N-terminal amino acid adjacent to a proline residue. The gene product may play a role in degradation and maturation of tachykinins neuropeptides and peptide hormones. Alternative splicing results in multiple transcript variants.[provided by RefSeq Nov 2009]

Gene Symbol:   XPNPEP1
Gene Name:   X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
Aliases:   APP1, SAMP, XPNPEP, XPNPEPL, XPNPEPL1
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000108039
Entrez:   7511
Chromosome Mapping:   10q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.490000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download