PrimePCR™ SYBR® Green Assay: ZFYVE16, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028314

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZFYVE16, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZFYVE16, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012125
Assay Design:   Intron-spanning
Chromosome Location:   5:79739019-79741158question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000338008 ENST00000510158 ENST00000505560

Gene Information

The ZFYVE16 gene encodes endofin an endosomal protein implicated in regulating membrane trafficking. It is characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain positioned in the middle of the molecule (Seet et al. 2004 [PubMed 14613930]).[supplied by OMIM Mar 2008]

Gene Symbol:   ZFYVE16
Gene Name:   zinc finger, FYVE domain containing 16
Aliases:   DKFZp686E13162, ENDOFIN, KIAA0305
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000039319
Entrez:   9765
Chromosome Mapping:   5q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.660000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download