This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028299
PrimePCR™ PreAmp for SYBR® Green Assay: NMNAT2, Human
PrimePCR™ Template for SYBR® Green Assay: NMNAT2, Human
This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member which is localized to the nucleus and is ubiquitously expressed; this enzyme is cytoplasmic and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.