PrimePCR™ SYBR® Green Assay: PRPF31, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028265

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PRPF31, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PRPF31, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012013
Assay Design:   Intron-spanning
Chromosome Location:   19:54625298-54626887question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000321030 ENST00000445811 ENST00000445124 ENST00000447810 ENST00000391755 ENST00000263436 ENST00000419967

Gene Information

This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex activation occurs.[provided by RefSeq Jan 2009]

Gene Symbol:   PRPF31
Gene Name:   PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
Aliases:   DKFZp566J153, NY-BR-99, PRP31, RP11
RefSeq:   NC_000019.9 NG_009759.1 NT_011109.16
Ensembl:   ENSG00000105618
Entrez:   26121
Chromosome Mapping:   19q13.42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.610000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download