PrimePCR™ SYBR® Green Assay: LRRC8A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028259

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LRRC8A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LRRC8A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012003
Assay Design:   Intron-spanning
Chromosome Location:   9:131671546-131678465question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000372600 ENST00000372599 ENST00000259324

Gene Information

This gene encodes a protein belonging to the leucine-rich repeat family of proteins which are involved in diverse biological processes including cell adhesion cellular trafficking and hormone-receptor interactions. This family member is a putative four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia an immunodeficiency disease resulting from defects in B cell maturation. Multiple alternatively spliced transcript variants which encode the same protein have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   LRRC8A
Gene Name:   leucine rich repeat containing 8 family, member A
Aliases:   AGM5, FLJ10337, FLJ41617, KIAA1437, LRRC8
RefSeq:   NC_000009.11 NT_008470.19 NG_009630.1
Ensembl:   ENSG00000136802
Entrez:   56262
Chromosome Mapping:   9q34.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.800000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download