This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028259
PrimePCR™ PreAmp for SYBR® Green Assay: LRRC8A, Human
PrimePCR™ Template for SYBR® Green Assay: LRRC8A, Human
This gene encodes a protein belonging to the leucine-rich repeat family of proteins which are involved in diverse biological processes including cell adhesion cellular trafficking and hormone-receptor interactions. This family member is a putative four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia an immunodeficiency disease resulting from defects in B cell maturation. Multiple alternatively spliced transcript variants which encode the same protein have been identified for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.