This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028255
PrimePCR™ PreAmp for SYBR® Green Assay: PUS10, Human
PrimePCR™ Template for SYBR® Green Assay: PUS10, Human
Pseudouridination the isomerization of uridine to pseudouridine is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases such as PUS10 catalyze pseudouridination of structural RNAs including transfer ribosomal and splicing RNAs. These enzymes also act as RNA chaperones facilitating the correct folding and assembly of tRNAs (McCleverty et al. 2007 [PubMed 17900615]).[supplied by OMIM May 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.