PrimePCR™ SYBR® Green Assay: PUS10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028255

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011991
Assay Design:   Intron-spanning
Chromosome Location:   2:61169466-61172182question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000316752 ENST00000407787

Gene Information

Pseudouridination the isomerization of uridine to pseudouridine is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases such as PUS10 catalyze pseudouridination of structural RNAs including transfer ribosomal and splicing RNAs. These enzymes also act as RNA chaperones facilitating the correct folding and assembly of tRNAs (McCleverty et al. 2007 [PubMed 17900615]).[supplied by OMIM May 2009]

Gene Symbol:   PUS10
Gene Name:   pseudouridylate synthase 10
Aliases:   CCDC139, DOBI, FLJ32312, MGC126729, MGC126755
RefSeq:   NG_008665.1 NC_000002.11 NT_022184.15
Ensembl:   ENSG00000162927
Entrez:   150962
UniGene:   Hs.368348
Chromosome Mapping:   2p16.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 34.200000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download