PrimePCR™ SYBR® Green Assay: WNK2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028246

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: WNK2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: WNK2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011963
Assay Design:   Intron-spanning
Chromosome Location:   9:96055378-96060213question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000297954 ENST00000432730 ENST00000411624 ENST00000448251 ENST00000453718 ENST00000395477 ENST00000395475 ENST00000349097 ENST00000427277 ENST00000356055

Gene Information

The protein encoded by this gene is a cytoplasmic serine-threonine kinase that contains cysteine in place of the lysine found at the conserved ATP-binding location in subdomain II of protein kinases. Since this protein does have kinase activity it is possible that another lysine in the kinase subdomain I can substitute for the missing conserved lysine. [provided by RefSeq Jul 2008]

Gene Symbol:   WNK2
Gene Name:   WNK lysine deficient protein kinase 2
Aliases:   KIAA1760, NY-CO-43, P/OKcl.13, PRKWNK2, SDCCAG43
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000165238
Entrez:   65268
Chromosome Mapping:   9q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.010000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download