PrimePCR™ SYBR® Green Assay: RNF32, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011819
Assay Design:   Intron-spanning
Chromosome Location:   7:156436495-156437250question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000404282 ENST00000439609 ENST00000317955 ENST00000405335 ENST00000311822 ENST00000392741 ENST00000432459 ENST00000392743 ENST00000343665 ENST00000392740

Gene Information

The protein encoded by this gene contains two RING ring finger motifs. RING finger motifs are present in a variety of functionally distinct proteins and are known to be involved in protein-DNA or protein-protein interactions. This gene was found to be expressed during spermatogenesis most likely in spermatocytes and/or in spermatids. Several alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jan 2011]

Gene Symbol:   RNF32
Gene Name:   ring finger protein 32
Aliases:   FKSG33, HSD15
RefSeq:   NC_000007.13 NT_007741.14
Ensembl:   ENSG00000105982
Entrez:   140545
UniGene:   Hs.446194
Chromosome Mapping:   7q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.010000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download