PrimePCR™ SYBR® Green Assay: ILKAP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ILKAP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ILKAP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011777
Assay Design:   Intron-spanning
Chromosome Location:   2:239093856-239096817question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000254654 ENST00000457149

Gene Information

The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1) a regulator of integrin mediated signaling and regulate the kinase activity of ILK. Through the interaction with ILK this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta) and thus participate in Wnt signaling pathway. [provided by RefSeq Jul 2008]

Gene Symbol:   ILKAP
Gene Name:   integrin-linked kinase-associated serine/threonine phosphatase
Aliases:   DKFZp434J2031, FLJ10181, MGC4846, PP2C-DELTA
RefSeq:   NC_000002.11 NT_005120.16
Ensembl:   ENSG00000132323
Entrez:   80895
Chromosome Mapping:   2q37.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.130000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download