This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028171
PrimePCR™ PreAmp for SYBR® Green Assay: COL21A1, Human
PrimePCR™ Template for SYBR® Green Assay: COL21A1, Human
This gene encodes the alpha chain of type XXI collagen a member of the FACIT collagen family (fibril-associated collagens with interrupted helices). Type XXI collagen is localized to tissues containing type I collagen so like other members of this collagen family it may serve to maintain the integrity of the extracellular matrix. An alternatively spliced transcript variant has been described but its full-length nature has yet to be determined. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.